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Western blot All lanes: AGPAT2 antibody at 1.09ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 31, 28 kDa Observed band size: 31 kDa
Western blot All lanes: AGPAT2 antibody at 1.09ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 31, 28 kDa Observed band size: 31 kDa
Western blot All lanes: AGPAT2 antibody at 1.09ug/ml + Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 31, 28 kDa Observed band size: 31 kDa

AGPAT2 Antibody

CSB-PA001450ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAGPAT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AGPAT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10555
  • Target name
    AGPAT2
  • Target description
    1-acylglycerol-3-phosphate O-acyltransferase 2
  • Target synonyms
    1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta); 1-acyl-sn-glycerol-3-phosphate acyltransferase beta; 1-AGP acyltransferase 2; 1-AGPAT 2; 1-AGPAT2; BSCL; BSCL1; LPAAB; LPAAT-beta; lysophosphatidic acid acyltransferase-beta; testicular tissue protein Li 143
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15120
  • Protein Name
    1-acyl-sn-glycerol-3-phosphate acyltransferase beta
  • Scientific Description
    Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AGPAT2 using anti-AGPAT2 antibody (A04744-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: rat pancreas tissue lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGPAT2 antigen affinity purified polyclonal antibody (Catalog # A04744-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGPAT2 at approximately 33 kDa. The expected band size for AGPAT2 is at 33 kDa.
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WB analysis of Jurkat cells using GTX46672 AGPAT2 antibody at 5.0microg/ml. Lane A : marker Lane B : Jurkat cells
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