AHA-1 antibody

GeneTex

AHA-1 antibody

9

WB: 1 microg/mL. ELISA: 1:35000-1:185000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

AHSA1

activator of HSP90 ATPase activity 1

AHA1, C14orf3, hAha1, p38, activator of 90 kDa heat shock protein ATPase homolog 1, AHA1, activator of heat shock 90kDa protein ATPase homolog 1

Rabbit

IgG

Activator of 90 kDa heat shock protein ATPase homolog 1

Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo. The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 molecular chaperone. Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle. Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone. Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER). Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.

-20°C or -80°C,2°C to 8°C

12352203