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Confocal immunofluorescence analysis of U2OS cells using AKAP8 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using AKAP8 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U2OS cells using AKAP8 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

AKAP8 Polyclonal Antibody

E-AB-62339
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    AKAP8 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a member of the A-kinase anchor protein family. A-kinase anchor proteins are scaffold proteins that contain a binding domain for the RI/RII subunit of protein kinase A (PKA) and recruit PKA and other signaling molecules to specific subcellular locations. This gene encodes a nuclear A-kinase anchor protein that binds to the RII alpha subunit of PKA and may play a role in chromosome condensation during mitosis by targeting PKA and the condensin complex to chromatin. A pseudogene of this gene is located on the short arm of chromosome 9.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKAP 95/AKAP8 using anti-AKAP 95/AKAP8 antibody (A05133-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKAP 95/AKAP8 antigen affinity purified polyclonal antibody (Catalog # A05133-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKAP 95/AKAP8 at approximately 92 kDa. The expected band size for AKAP 95/AKAP8 is at 76 kDa.
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