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AKT3 antibody

10R-3271
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    AKT3 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Immunoprecipitating AKT3 in mouse brain whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA15929A0Rb in mouse brain whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA15929A0Rb (6ug) + Mouse brain whole cell lysate (500ug) Lane 3: Mouse brain whole cell lysate (10ug)
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Figure 1. Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3 mouse brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (Catalog # A00520-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 60 kDa. The expected band size for AKT3 is at 56 kDa.
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Immunohistochemical staining of human cerebral cortex shows strong nuclear and cytoplasmic positivity in neurons.
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