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Goat anti-AKT3

EB06562
Everest Biotech
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAKT3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-AKT3 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID10000
  • Target name
    AKT3
  • Target description
    AKT serine/threonine kinase 3
  • Target synonyms
    MPPH; MPPH2; PKB gamma; PKBG; PKB-GAMMA; PRKBG; RAC-gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; STK-2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_005456.1; NP_859029.1. Purification: Antigen affinity purified. Names and symbols: AKT3; PKBG; PRKBG; STK-2; RAC-gamma; RAC-PK-gamma; DKFZP434N0250; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); protein kinase B gamma; serine threonine protein kinase, Akt-3; RAC-gamma serine/threonine protein kinase; PKB-GAMMA
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3 mouse brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (Catalog # A00520-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 60 kDa. The expected band size for AKT3 is at 56 kDa.
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