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Immunoprecipitating AKT3 in mouse brain whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA15929A0Rb in mouse brain whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA15929A0Rb (6ug) + Mouse brain whole cell lysate (500ug) Lane 3: Mouse brain whole cell lysate (10ug)
Immunoprecipitating AKT3 in mouse brain whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA15929A0Rb in mouse brain whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA15929A0Rb (6ug) + Mouse brain whole cell lysate (500ug) Lane 3: Mouse brain whole cell lysate (10ug)
Immunoprecipitating AKT3 in mouse brain whole cell lysate Lane 1: Rabbit control IgG (1ug) instead of CSB-PA15929A0Rb in mouse brain whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: CSB-PA15929A0Rb (6ug) + Mouse brain whole cell lysate (500ug) Lane 3: Mouse brain whole cell lysate (10ug)

AKT3 Antibody

CSB-PA15929A0RB
Cusabio
ApplicationsImmunoPrecipitation, ELISA
Product group Antibodies
TargetAKT3
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Overview

  • Supplier
    Cusabio
  • Product Name
    AKT3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10000
  • Target name
    AKT3
  • Target description
    AKT serine/threonine kinase 3
  • Target synonyms
    MPPH; MPPH2; PKB gamma; PKBG; PKB-GAMMA; PRKBG; RAC-gamma; RAC-gamma serine/threonine protein kinase; RAC-gamma serine/threonine-protein kinase; RAC-PK-gamma; STK-2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y243
  • Protein Name
    RAC-gamma serine/threonine-protein kinase
  • Scientific Description
    AKT3 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT3 is the least studied AKT isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. AKT3 isoform may also be the key molecule in up-regulation and down-regulation of MMP13 via IL13. Required for the coordination of mitochondrial biogenesis with growth factor-induced increases in cellular energy demands. Down-regulation by RNA interference reduces the expression of the phosphorylated form of BAD, resulting in the induction of caspase-dependent apoptosis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AKT3 using anti-AKT3 antibody (A00520-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3 mouse brain tissue lysates, Lane 4: mouse lung tissue lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U-87MG whole cell lysates, Lane 8: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AKT3 antigen affinity purified polyclonal antibody (Catalog # A00520-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AKT3 at approximately 60 kDa. The expected band size for AKT3 is at 56 kDa.
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