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ProductsBack/ALDH1L1 Mouse Monoclonal Antibody [Clone ID: OTI5G8]
Anti-ALDH1L1 mouse monoclonal antibody (TA501870) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1 (RC213720).
Anti-ALDH1L1 mouse monoclonal antibody (TA501870) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1 (RC213720).
Anti-ALDH1L1 mouse monoclonal antibody (TA501870) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ALDH1L1 (RC213720).

ALDH1L1 Mouse Monoclonal Antibody [Clone ID: OTI5G8]

CF501870
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetALDH1L1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    ALDH1L1 Mouse Monoclonal Antibody [Clone ID: OTI5G8]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5G8
  • Gene ID10840
  • Target name
    ALDH1L1
  • Target description
    aldehyde dehydrogenase 1 family member L1
  • Target synonyms
    10-FTHFDH, 10-fTHF, FDH, FTHFD, cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-formyltetrahydrofolate dehydrogenase, MER57B-ALDH1L1
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDO75891
  • Protein Name
    Cytosolic 10-formyltetrahydrofolate dehydrogenase
  • Scientific Description
    Carrier-free (BSA/glycerol-free) ALDH1L1 mouse monoclonal antibody, clone OTI5G8 (formerly 5G8)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Immunohistochemical staining of human cerebral cortex, kidney, liver and lymph node using Anti-ALDH1L1 antibody HPA036900 (A) shows similar protein distribution across tissues to independent antibody HPA050139 (B).
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Figure 1. Western blot analysis of ALDH1L1 using anti-ALDH1L1 antibody (A04615-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEPG2 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: monkey liver tissue lysates, Lane 4: monkey kidney tissue lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH1L1 antigen affinity purified polyclonal antibody (Catalog # A04615-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH1L1 at approximately 97KD. The expected band size for ALDH1L1 is at 97KD.
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