Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230505_WB_23050918_741.webp "Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230616_WB_D_23062019_536.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230804_WB_23080901_450.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20240607_IHC-P_24061301_687.webp "Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20240607_IHC-P_M_24061301_196.webp "Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] detects Alkaline Phosphatase (Tissue Non-Specific) protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Alkaline Phosphatase (Tissue Non-Specific) stained by Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20240927_WB_TPM_watermark_24100318_441.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003]
GTX637904
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Mouse
TargetALPL
Overview
- SupplierGeneTex
- Product NameAlkaline Phosphatase (Tissue Non-Specific) antibody [HL2003]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2003
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID249
- Target nameALPL
- Target descriptionalkaline phosphatase, biomineralization associated
- Target synonymsAP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, liver/bone/kidney-type alkaline phosphatase, phosphoamidase, phosphocreatine phosphatase, tissue non-specific alkaline phosphatase, tissue-nonspecific ALP
- HostRabbit
- IsotypeIgG
- Protein IDP05186
- Protein NameAlkaline phosphatase, tissue-nonspecific isozyme
- Scientific DescriptionThis gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
- ReactivityCanine, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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