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alpha Actinin 4 antibody

70R-2835
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    alpha Actinin 4 antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, 293T whole cell lysate, Rat liver tissue All lanes: ACTN4 antibody at 3.3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 105, 80, 60 kDa Observed band size: 105 kDa
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Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using anti-alpha-Actinin 4 rabbit monoclonal antibody (clone RM287) at a 1:200 dilution.
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Figure 1. Western blot analysis of IRE1 using anti-IRE1 antibody (A00683-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human SK-OV-3 whole cell lysates, Lane 3: human PANC-1 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRE1 antigen affinity purified polyclonal antibody (Catalog # A00683-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRE1 at approximately 170KD. The expected band size for IRE1 is at 110KD.
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Immunocytochemical analysis of Actinin-4 distribution in rat kidney using anti-alpha-Actinin-4, pAb (IG-701) (AG-25T-0107). Glomeruli are strongly labeled (frozen sections of formaldehyde perfused tissue (dilution 1:1600)).
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References
GeneTex
alpha Actinin 4 antibody [C2C3], C-termGTX101669
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetACTN4
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