alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 whole cell lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX101669/GTX101669_40205_IHC_M_w_23060100_697.webp "alpha Actinin 4 antibody [C2C3], C-term detects alpha Actinin 4 protein on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:500.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101669/GTX101669_40205_WB_R_w_23060100_553.webp "alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg PC-12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101669/GTX101669_40205_WB_w_23060100_743.webp "alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract D. 30 μg HepG2 whole cell lysate/extract E. 30 μg A375 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody [C2C3], C-term (GTX101669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101669/GTX101669_44559_20220114_WB_24052802_456.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Actinin 4 antibody [C2C3], C-term (GTX101669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
alpha Actinin 4 antibody [C2C3], C-term detects ACTN4 protein by western blot analysis. A. 30 μg Neuro2A whole cell lysate/extract B. 30 μg GL261 whole cell lysate/extract C. 30 μg C8D30 whole cell lysate/extract D. 30 μg NIH-3T3 whole cell lysate/extract E. 30 μg BCL-1 whole cell lysate/extract F. 30 μg Raw264.7 whole cell lysate/extract G. 30 μg C2C12 whole cell lysate/extract 7.5% SDS-PAGE alpha Actinin 4 antibody [C2C3], C-term (GTX101669) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Actinin 4 antibody [C2C3], C-term
GTX101669
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetACTN4
Overview
- SupplierGeneTex
- Product Namealpha Actinin 4 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.32 mg/ml
- ConjugateUnconjugated
- Gene ID81
- Target nameACTN4
- Target descriptionactinin alpha 4
- Target synonymsACTININ-4, FSGS, FSGS1, alpha-actinin-4, focal segmental glomerulosclerosis 1, non-muscle alpha-actinin 4
- HostRabbit
- IsotypeIgG
- Protein IDO43707
- Protein NameAlpha-actinin-4
- Scientific DescriptionAlpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Ho CH, Yang HH, Su SH, et al. α-Actinin 4 Links Vasopressin Short-Term and Long-Term Regulation of Aquaporin-2 in Kidney Collecting Duct Cells. Front Physiol. 2021,12:725172. doi: 10.3389/fphys.2021.725172Read this paper
- Yeh SH, Lin CH, Yang YJ, et al. Higher senescence associated secretory phenotype and lower defense mediator in urinary extracellular vesicles of elders with and without Parkinson disease. Sci Rep. 2021,11(1):15783. doi: 10.1038/s41598-021-95062-yRead this paper
- Hiraga C, Yamamoto S, Hashimoto S, et al. Pentapartite fractionation of particles in oral fluids by differential centrifugation. Sci Rep. 2021,11(1):3326. doi: 10.1038/s41598-021-82451-6Read this paper
- Takov K, Yellon DM, Davidson SM. Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential. J Extracell Vesicles. 2019,8(1):1560809. doi: 10.1080/20013078.2018.1560809Read this paper
- Ageta H, Ageta-Ishihara N, Hitachi K, et al. UBL3 modification influences protein sorting to small extracellular vesicles. Nat Commun. 2018,9(1):3936. doi: 10.1038/s41467-018-06197-yRead this paper
- Durrieu L, Bharadwaj A, Waisman DM. Analysis of the thrombotic and fibrinolytic activities of tumor cell-derived extracellular vesicles. Blood Adv. 2018,2(10):1054-1065. doi: 10.1182/bloodadvances.2017015479Read this paper
- Choi W, Acharya BR, Peyret G, et al. Remodeling the zonula adherens in response to tension and the role of afadin in this response. J Cell Biol. 2016,213(2):243-60. doi: 10.1083/jcb.201506115Read this paper
- Kowal J, Arras G, Colombo M, et al. Proteomic comparison defines novel markers to characterize heterogeneous populations of extracellular vesicle subtypes. Proc Natl Acad Sci U S A. 2016,113(8):E968-77. doi: 10.1073/pnas.1521230113Read this paper
Datasheet
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