Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100034/GTX100034_43040_20191108_IHC-P-FL_M_1_w_23053123_957.webp "alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100034/GTX100034_43040_20191108_IHC-P-FL_R_1_w_23053123_542.webp "alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat cervix. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100034/GTX100034_43040_20191108_IHC-P-FL_M_w_23053123_849.webp "alpha Smooth Muscle Actin antibody detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. Green: alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody (GTX100034) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
alpha Smooth Muscle Actin antibody
GTX100034
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetACTA2
Overview
- SupplierGeneTex
- Product Namealpha Smooth Muscle Actin antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.14 mg/ml
- ConjugateUnconjugated
- Gene ID59
- Target nameACTA2
- Target descriptionactin alpha 2, smooth muscle
- Target synonymsACTSA, SMDYS, actin, aortic smooth muscle, actin, alpha 2, smooth muscle, aorta, alpha-cardiac actin, cell growth-inhibiting gene 46 protein
- HostRabbit
- IsotypeIgG
- Protein IDP62736
- Protein NameActin, aortic smooth muscle
- Scientific DescriptionThe protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
- ReactivityHuman, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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![FACS analysis of L929 cells using GTX17501 alpha Smooth Muscle Actin antibody [9H15] (PE).](https://www.genetex.com/upload/website/prouct_img/normal/GTX17501/GTX17501_20191203_FACS_0_w_23060620_472.webp)
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![IHC-P analysis of human small bowel tissue using GTX44499 alpha Smooth Muscle Actin antibody [αsm-1]. Note cytoplasmic staining of the muscularis mucosa, vascular walls and smooth muscle fibers in the lamina propria.](https://www.genetex.com/upload/website/prouct_img/normal/GTX44499/GTX44499_20200811_IHC-P_5_w_23060820_700.webp)
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