More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/AMFR antibody
IHC-P analysis of human placenta tissue using GTX04315 AMFR antibody. Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0) Dilution : 1 μg/ml
IHC-P analysis of human placenta tissue using GTX04315 AMFR antibody. Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0) Dilution : 1 μg/ml
IHC-P analysis of human placenta tissue using GTX04315 AMFR antibody. Antigen retrieval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0) Dilution : 1 μg/ml

AMFR antibody

GTX04315
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAMFR
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    AMFR antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. ICC/IF: 5 microg/ml. IHC-P: 0.5-1microg/ml. FCM: 1-3microg/1x106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID267
  • Target name
    AMFR
  • Target description
    autocrine motility factor receptor
  • Target synonyms
    GP78, RNF45, SPG89, E3 ubiquitin-protein ligase AMFR, RING finger protein 45, RING-type E3 ubiquitin transferase AMFR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKV5
  • Protein Name
    E3 ubiquitin-protein ligase AMFR
  • Scientific Description
    This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
AMFR AntibodyCAB3731
ReactivityHuman
TargetAMFR
  • SizePrice
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: AMFR antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
Product group Antibodies
Cusabio
AMFR AntibodyCSB-PA333164LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetAMFR
  • SizePrice
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Product group Antibodies
Atlas Antibodies
Anti-AMFR AntibodyHPA029018
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetAMFR
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
GP78 / AMFR AntibodyLS-C669761
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetAMFR
  • SizePrice
Product group Antibodies
Biomatik
AMFR Polyclonal AntibodyCAC15127
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetAMFR
  • SizePrice
Figure 1. Western blot analysis of AMFR using anti-AMFR antibody (PB10039). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMFR antigen affinity purified polyclonal antibody (Catalog # PB10039) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMFR at approximately 73 kDa. The expected band size for AMFR is at 73 kDa.
Product group Antibodies
Boster Bio
Anti-AMFR Antibody Picoband(r)PB10039-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetAMFR
  • SizePrice
Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2865] (GTX640142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AMFR antibody [HL2865]GTX640142
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetAMFR
  • SizePrice
Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
AMFR antibody [HL2866]GTX640143
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse
TargetAMFR
  • SizePrice
Product group Antibodies
RayBiotech
Anti-AMFR Antibody144-65732
ApplicationsWestern Blot
ReactivityHuman
TargetAMFR
  • SizePrice
Product group Antibodies
Bioss
RNF45 Polyclonal AntibodyBS-6511R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Human, Mouse, Rabbit, Rat
TargetAMFR
  • SizePrice