![Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640143/GTX640143_T-45362_20240607_WB_M_liver_24061301_878.webp)
Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
AMFR antibody [HL2866]
GTX640143
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetAMFR
Overview
- SupplierGeneTex
- Product NameAMFR antibody [HL2866]
- Delivery Days Customer7
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2866
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID267
- Target nameAMFR
- Target descriptionautocrine motility factor receptor
- Target synonymsGP78, RNF45, SPG89, E3 ubiquitin-protein ligase AMFR, RING finger protein 45, RING-type E3 ubiquitin transferase AMFR
- HostRabbit
- IsotypeIgG
- Protein IDQ9UKV5
- Protein NameE3 ubiquitin-protein ligase AMFR
- Scientific DescriptionThis locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![AMFR antibody [HL2866] detects AMFR protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: AMFR stained by AMFR antibody [HL2866] (GTX640143) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). AMFR antibody [HL2866] detects AMFR protein by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: AMFR stained by AMFR antibody [HL2866] (GTX640143) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX640143/GTX640143_T-45362_20240524_ICC_IF_24070822_759.webp)
![Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Unboiled HepG2 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2866] (GTX640143) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640143/GTX640143_45488_20240802_WB_Fraction_24082301_995.webp)




![Boiled and unboiled mouse tissue extract (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMFR antibody [HL2865] (GTX640142) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX640142/GTX640142_T-45362_20240412_WB_M_liver_24041523_454.webp)

