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Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA12639A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA12639A0Rb at dilution of 1:100
Immunohistochemistry of paraffin-embedded human ovarian cancer using CSB-PA12639A0Rb at dilution of 1:100

AMH Antibody

CSB-PA12639A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAMH
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Overview

  • Supplier
    Cusabio
  • Product Name
    AMH Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID268
  • Target name
    AMH
  • Target description
    anti-Mullerian hormone
  • Target synonyms
    anti-Muellerian hormone; MIF; MIS; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian inhibiting factor; Mullerian inhibiting substance
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03971
  • Protein Name
    Muellerian-inhibiting factor
  • Scientific Description
    This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AMH using anti-AMH antibody (A00763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole Cell lysates, Lane 2: COLO320 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody (Catalog # A00763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
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