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Anti-anti-Mullerian hormone [AMH-1]

AB02789-10.0-BT
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetAMH
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-anti-Mullerian hormone [AMH-1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The binding characterization of this antibody for recombinant AMH was done using ELISA. This antibody was used as a capture antibody in combination with another anti-AMH antibody AMH-2 as labelling antibody for a double antibody sandwich ELISA (CN110407937).
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AMH-1
  • Gene ID268
  • Target name
    AMH
  • Target description
    anti-Mullerian hormone
  • Target synonyms
    anti-Muellerian hormone; MIF; MIS; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian inhibiting factor; Mullerian inhibiting substance
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP03971
  • Protein Name
    Muellerian-inhibiting factor
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMH using anti-AMH antibody (A00763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole Cell lysates, Lane 2: COLO320 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody (Catalog # A00763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
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