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AMH Polyclonal Antibody

BS-43520R
Bioss
ApplicationsWestern Blot
Product group Antibodies
TargetAMH
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Overview

  • Supplier
    Bioss
  • Product Name
    AMH Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID268
  • Target name
    AMH
  • Target description
    anti-Mullerian hormone
  • Target synonyms
    MIF, MIS, muellerian-inhibiting factor, Mullerian inhibiting factor, Mullerian inhibiting substance, anti-Muellerian hormone, muellerian-inhibiting substance
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03971
  • Protein Name
    Muellerian-inhibiting factor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMH using anti-AMH antibody (A00763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole Cell lysates, Lane 2: COLO320 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody (Catalog # A00763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
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