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Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AMH antibody (GTX129593) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AMH antibody (GTX129593) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AMH antibody (GTX129593) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AMH antibody

GTX129593
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityFeline, Human, Mouse
TargetAMH
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Overview

  • Supplier
    GeneTex
  • Product Name
    AMH antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID268
  • Target name
    AMH
  • Target description
    anti-Mullerian hormone
  • Target synonyms
    MIF, MIS, muellerian-inhibiting factor, Mullerian inhibiting factor, Mullerian inhibiting substance, anti-Muellerian hormone, muellerian-inhibiting substance
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP03971
  • Protein Name
    Muellerian-inhibiting factor
  • Scientific Description
    Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq]
  • Reactivity
    Feline, Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AMH using anti-AMH antibody (A00763). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: 293T whole Cell lysates, Lane 2: COLO320 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMH antigen affinity purified polyclonal antibody (Catalog # A00763) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMH4 at approximately 65KD. The expected band size for AMH is at 59KD.
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