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FACS analysis of LNCaP cells using GTX57585 Amphiregulin antibody. Cell Number: 1 x 10? cells Primary antibody: Red line Antibody amount: 2-5 microg
FACS analysis of LNCaP cells using GTX57585 Amphiregulin antibody. Cell Number: 1 x 10? cells Primary antibody: Red line Antibody amount: 2-5 microg
FACS analysis of LNCaP cells using GTX57585 Amphiregulin antibody. Cell Number: 1 x 10? cells Primary antibody: Red line Antibody amount: 2-5 microg

Amphiregulin antibody [AT1D10]

GTX57585
GeneTex
ApplicationsFlow Cytometry, Western Blot
DownloadDatasheet
Product group Antibodies
TargetAREG
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Overview

  • Supplier
    GeneTex
  • Product Name
    Amphiregulin antibody [AT1D10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:5000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT1D10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID374
  • Target name
    AREG
  • Target description
    amphiregulin
  • Target synonyms
    amphiregulin; amphiregulin B; AR; AREGB; colorectum cell-derived growth factor; CRDGF; schwannoma-derived growth factor; SDGF
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP15514
  • Protein Name
    Amphiregulin
  • Scientific Description
    The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Amphiregulin/AREG using anti-Amphiregulin/AREG antibody (A01787-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiregulin/AREG antigen affinity purified polyclonal antibody (Catalog # A01787-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiregulin/AREG at approximately 45 kDa. The expected band size for Amphiregulin/AREG is at 28 kDa.
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