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Angiopoietin 2 antibody

10-2265
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    Angiopoietin 2 antibody
  • Delivery Days Customer
    7
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody (Catalog # A00370-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPT2 at approximately 66KD. The expected band size for ANGPT2 is at 57KD.
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