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Western Blot Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse brain tissue All lanes: ANGPT2 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 51 kDa Observed band size: 57, 51 kDa
Western Blot Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse brain tissue All lanes: ANGPT2 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 51 kDa Observed band size: 57, 51 kDa
Western Blot Positive WB detected in: Rat brain tissue, Rat kidney tissue, Mouse brain tissue All lanes: ANGPT2 antibody at 3.2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 57, 51 kDa Observed band size: 57, 51 kDa

ANGPT2 Antibody

CSB-PA05749A0RB
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetANGPT2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANGPT2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID285
  • Target name
    ANGPT2
  • Target description
    angiopoietin 2
  • Target synonyms
    AGPT2; ANG2; angiopoietin-2; angiopoietin-2a; angiopoietin-2B; LMPHM10; Tie2-ligand
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15123
  • Protein Name
    Angiopoietin-2
  • Scientific Description
    Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody (Catalog # A00370-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPT2 at approximately 66KD. The expected band size for ANGPT2 is at 57KD.
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