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Angiopoietin 2 antibody

GTX100928
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetANGPT2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Angiopoietin 2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1.41 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID285
  • Target name
    ANGPT2
  • Target description
    angiopoietin 2
  • Target synonyms
    AGPT2, ANG2, LMPHM10, angiopoietin-2, Tie2-ligand, angiopoietin-2B, angiopoietin-2a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15123
  • Protein Name
    Angiopoietin-2
  • Scientific Description
    The protein encoded by this gene is an antagonist of angiopoietin 1 (ANGPT1) and endothelial TEK tyrosine kinase (TIE-2, TEK). The encoded protein disrupts the vascular remodeling ability of ANGPT1 and may induce endothelial cell apoptosis. Three transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Yao Y, Cai Y, Sui A, et al. Etanercept as a TNF-alpha inhibitor depresses experimental retinal neovascularization. Graefes Arch Clin Exp Ophthalmol. 2021,259(3):661-671. doi: 10.1007/s00417-020-04956-6
    Read this paper
  • Gu J, Zhang Y, Han Z, et al. Targeting the ERβ/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC. Cell Death Dis. 2020,11(5):367. doi: 10.1038/s41419-020-2486-0
    Read this paper
  • Chen CM, Juan SH, Chou HC. Hyperglycemia activates the renin-angiotensin system and induces epithelial-mesenchymal transition in streptozotocin-induced diabetic kidneys. J Renin Angiotensin Aldosterone Syst. 2018,19(3):1470320318803009. doi: 10.1177/1470320318803009
    Read this paper
  • Fischer D, Seifen C, Baer P, et al. The Fibrin Cleavage Product Bβ(15-42) Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury. Front Pharmacol. 2018,9:369. doi: 10.3389/fphar.2018.00369
    Read this paper

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Figure 1. Western blot analysis of ANGPT2 using anti-ANGPT2 antibody (A00370-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse spleen tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPT2 antigen affinity purified polyclonal antibody (Catalog # A00370-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPT2 at approximately 66KD. The expected band size for ANGPT2 is at 57KD.
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