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ProductsBack/Angiotensin Converting Enzyme 2 (ACE2) Rabbit Polyclonal Antibody
Western blot analysis of human kidney lysate probed with Rabbit anti Human ACE2 antibody
Western blot analysis of human kidney lysate probed with Rabbit anti Human ACE2 antibody
Western blot analysis of human kidney lysate probed with Rabbit anti Human ACE2 antibody

Angiotensin Converting Enzyme 2 (ACE2) Rabbit Polyclonal Antibody

AP05885PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetACE2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    Angiotensin Converting Enzyme 2 (ACE2) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID59272
  • Target name
    ACE2
  • Target description
    angiotensin converting enzyme 2
  • Target synonyms
    ACEH, angiotensin-converting enzyme 2, ACE-related carboxypeptidase, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin I converting enzyme 2, angiotensin-converting enzyme homolog, angiotensin-converting enzyme-related carboxypeptidase, metalloprotease MPROT15, peptidyl-dipeptidase A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BYF1
  • Protein Name
    Angiotensin-converting enzyme 2
  • Scientific Description
    Angiotensin Converting Enzyme 2 (ACE2) rabbit polyclonal antibody, Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ACE2 using anti-ACE2 antibody (A00756-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACE2 antigen affinity purified polyclonal antibody (Catalog # A00756-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ACE2 at approximately 120 kDa. The expected band size for ACE2 is at 93 kDa.
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Anti-ACE2 Antibody Picoband(r)A00756-3-CARRIER-FREE
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TargetACE2
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Immunohistochemistry analysis in human kidney and tonsil tissues using AMAb91259 antibody. Corresponding ACE2 RNA-seq data are presented for the same tissues.
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Western blot analysis using anti-ACE2 (human), pAb (Prod. No. AG-25A-0042) at 1:2000 dilution. 1: Human ACE2 Fc-protein. 2: Human ACE2 (ED) (FLAG®-tagged).
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