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ProductsBack/Anterior Gradient 2 antibody
AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGR2 protein stained by AGR2 antibody (GTX130229) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGR2 protein stained by AGR2 antibody (GTX130229) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
AGR2 antibody detects AGR2 protein at cytoplasm by immunofluorescent analysis. Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: AGR2 protein stained by AGR2 antibody (GTX130229) diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 μm.

Anterior Gradient 2 antibody

GTX130229
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetAGR2
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Overview

  • Supplier
    GeneTex
  • Product Name
    Anterior Gradient 2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10551
  • Target name
    AGR2
  • Target description
    anterior gradient 2, protein disulphide isomerase family member
  • Target synonyms
    AG-2, AG2, GOB-4, HAG-2, HEL-S-116, HPC8, PDIA17, RIFTD, XAG-2, anterior gradient protein 2 homolog, anterior gradient homolog 2, epididymis secretory protein Li 116, protein disulfide isomerase family A, member 17, secreted cement gland homolog, secreted cement gland protein XAG-2 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95994
  • Protein Name
    Anterior gradient protein 2 homolog
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AGR2 using anti-AGR2 antibody (A02922-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T47D whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat small intestine tissue lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGR2 antigen affinity purified polyclonal antibody (Catalog # A02922-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGR2 at approximately 17 kDa. The expected band size for AGR2 is at 20 kDa.
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