Anti-Arg-2 [CPR02]

Absolute Antibody

Anti-Arg-2 [CPR02]

7

The antibody is specific for Arg-2. Arg-2 is required for MUC2 post-transcriptional synthesis and secretion and, it may play a role in the production of mucus by intestinal cells.

The scFv fragment of the antibody was able to bind the recombinant human Agr2 protein in ELISA analysis. The binding affinity of the scFv fragment to human Agr2 was measured by surface plasmon resonance (Kd= 4.6x10^-7 M). Immunofluorescence analysis of colon biopsy samples from a healthy donor was performed to verify the ability of CPR02 to recognize the human Agr2 antigen. Results showed the antibody revealed significant staining surrounding the crypts of the intestinal tissue. Further, the antibody was used for immunofluorescent staining of freshly frozen human biopsy samples of Ulcerative Colitis, the antibody produced a strong intensity signal in the colonic crypts region. The antibody was able to recognise the Agr2 antigen in vivo, as demonstrated by the results of the autoradiography of the complete intestinal tract of a mouse having colitis, injected with radiolabelled antibody (US20190309060A1).

ImmunoFluorescence, ELISA, Other Application

ELISA; IF; in vivo ; SPR

Research Use Only

Monoclonal

CPR02

AGR2

anterior gradient 2, protein disulphide isomerase family member

AG2; AG-2; anterior gradient homolog 2; anterior gradient protein 2 homolog; epididymis secretory protein Li 116; GOB-4; HAG-2; HEL-S-116; HPC8; PDIA17; protein disulfide isomerase family A, member 17; secreted cement gland homolog; secreted cement gland protein XAG-2 homolog; XAG-2

Rabbit

IgG

Anterior gradient protein 2 homolog

This full-length chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format for improved compatibility with existing reagents assays and techniques.

Human

Human

The antibody was isolated from the antibody library after three rounds of panning against residues 41-175 of recombinant human Agr2.

-20°C,2°C to 8°C

12352203