Anti-ACE2 [h11B11]

Absolute Antibody

Anti-ACE2 [h11B11]

9

This antibody binds the extracellular domain of the human ACE2 protein and cross reacts with cynomolgus monkey ACE2 protein. Angiotensin-converting enzyme 2 (ACE2) is a carboxypeptidase enzyme which catalyzes the cleavage of angiotensin I to angiotensin 1

This antibody can bind purified human ACE2 recombinant protein in an ELISA. The ability of this antibody to bind ACE2 and block its interaction with SARS-CoV-RBD and SARS-CoV-2-RBD was determined using flow cytometry. The binding affinity of the IgG1 version of this antibody to wild type human ACE2 was found to be Kd= 1.95 nM. This antibody was also capable of binding mutated versions of human ACE2 like I21T, K26R, N33D and D38E. This antibody does not inhibit the carboxypeptidase activity of hACE2 in vitro. This antibody showed strong inhibitory activity against SARS-CoV, SARS-CoV-2, and epidemic SARS-CoV-2 variant pseudo virus in vitro. When administered therapeutically or prophylactically in the hACE2 mouse model, this antibody alleviates and prevents SARS-CoV-2 replication and virus-induced pathological syndromes. This antibody also didnt exhibit any significant toxicity to cynomolgus monkey when administered in high doses (PMID: 34404805).

Flow Cytometry, ELISA, Neutralisation/Blocking

Research Use Only

Monoclonal

h11B11

ACE2

angiotensin converting enzyme 2

ACEH; ACE-related carboxypeptidase; angiotensin I converting enzyme (peptidyl-dipeptidase A) 2; angiotensin I converting enzyme 2; angiotensin-converting enzyme 2; angiotensin-converting enzyme homolog; angiotensin-converting enzyme-related carboxypeptidase; metalloprotease MPROT15; peptidyl-dipeptidase A; truncated angiotensin converting enzyme 2

Rabbit

IgG

Angiotensin-converting enzyme 2

Human, Monkey

-20°C,2°C to 8°C

12352203