Anti-ADAMTS5 [7B4]

Absolute Antibody

Anti-ADAMTS5 [7B4]

7

The antibody is specific for ADAMTS5 and it recognises an epitope within the catalytic and disintegrin domains (amino acids 262-576). ADAMTS-5 is a protease involved in cartilage degradation in human OA patients.

The antibody was characterized for binding affinity, species cross-reactivity and domain specificity. The antibody showed inhibitory potency (IC50) of 41.26 nM and humanization improved potency to 11.12 nM. The affinity of the original antibody and humanized version was measured, Kd= 0.280 nM and 0.205 nM respectively. Epitope mapping and selective binding to purified recombinant ADAMS5 were profiled by DELFIA. Selective binding of the antibody is demonstrated by immunocytochemistry using ADAMTS-5 and CHO cells. The antibody inhibited aggrecanolysis from human OA cartilage explants. The ability of the antibody to engage its target in vivo was assessed using labeled antibody in a preclinical OA model and whole body imaging was used to monitor biodistribution and cartilage penetration. The antibody treatment suppressed joint disease severity and mechanical allodynia. The crystal structure of the Fab fragment was determined (Larkin et al., 2015; PMID: 25800415).

ImmunoHistoChemistry, Neutralisation/Blocking, Other Application

Crystallography; IHC; in vivo; inhibition

Research Use Only

Monoclonal

7B4

ADAMTS5

ADAM metallopeptidase with thrombospondin type 1 motif 5

a disintegrin and metalloproteinase with thrombospondin motifs 11; A disintegrin and metalloproteinase with thrombospondin motifs 5; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2); ADAM-TS 11; ADAM-TS 5; ADAMTS11; ADAMTS-11; ADAM-TS5; ADAMTS-5; ADMP-2; aggrecanase-2

Human

IgG1

A disintegrin and metalloproteinase with thrombospondin motifs 5

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human, Mouse

Human, mouse

-20°C,2°C to 8°C

12352203