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Immunohistochemical staining of human prostate shows weak cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows weak cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows weak cytoplasmic positivity in glandular cells.

Anti-AMD1 Antibody

HPA029282
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAMD1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-AMD1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID262
  • Target name
    AMD1
  • Target description
    adenosylmethionine decarboxylase 1
  • Target synonyms
    ADOMETDC, AMD, SAMDC, S-adenosylmethionine decarboxylase proenzyme, S-adenosylmethionine decarboxylase 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17707
  • Protein Name
    S-adenosylmethionine decarboxylase proenzyme
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of AMD1 using anti-AMD1 antibody (A06146). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMD1 antigen affinity purified polyclonal antibody (Catalog # A06146) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMD1 at approximately 38KD. The expected band size for AMD1 is at 38KD.
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