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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.

Anti-ANXA6 Antibody

HPA009650
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetANXA6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ANXA6
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID309
  • Target name
    ANXA6
  • Target description
    annexin A6
  • Target synonyms
    67 kDa calelectrin; annexin A6; annexin VI (p68); annexin-6; ANX6; calcium-binding protein p68; calelectrin; calphobindin II; CBP68; chromobindin-20; CPB-II; lipocortin VI; p68; p70; testis secretory sperm-binding protein Li 198a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08133
  • Protein Name
    Annexin A6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Annexin VI/ANXA6 using anti-Annexin VI/ANXA6 antibody (A03735). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat lung tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Annexin VI/ANXA6 antigen affinity purified polyclonal antibody (Catalog # A03735) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Annexin VI/ANXA6 at approximately 68-76 kDa. The expected band size for Annexin VI/ANXA6 is at 76 kDa.
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