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Anti-ASCL1 Antibody

ORB2570170
Biorbyt
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetASCL1
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Overview

  • Supplier
    Biorbyt
  • Product Name
    Anti-ASCL1 Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Western blot, 0.25-0.5 microg/ml, Human, Mouse, Rat Immunohistochemistry, 2-5 microg/ml, Human Flow Cytometry (Fixed), 1-3 microg/1x106 cells, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 microg/ml
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    FC, IHC, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    APC (Allophycocyanin)
  • Gene ID429
  • Target name
    ASCL1
  • Target description
    achaete-scute family bHLH transcription factor 1
  • Target synonyms
    achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex-like 1; achaete-scute homolog 1; ASH1; ASH-1; bHLHa46; class A basic helix-loop-helix protein 46; HASH1; MASH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP50553
  • Protein Name
    Achaete-scute homolog 1
  • Scientific Description
    Anti-ASCL1 Antibody. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ASCL1 using anti-ASCL1 antibody (A03023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human NCL-H460 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASCL1 antigen affinity purified polyclonal antibody (Catalog # A03023-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASCL1 at approximately 30 kDa. The expected band size for ASCL1 is at 25 kDa.
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