Bio-Connect

Anti-ASCL1 [SC72.2]

AB02398-1.1-BT
Absolute Antibody
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetASCL1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-ASCL1 [SC72.2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody can bind ASCL1 in cell lysates via ELISA. This antibody was used for the immunohistochemical staining of PDX tumor sections to assess the expression of ASCL1 in tumor cells (US20190194319).
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SC72.2
  • Gene ID429
  • Target name
    ASCL1
  • Target description
    achaete-scute family bHLH transcription factor 1
  • Target synonyms
    achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex-like 1; achaete-scute homolog 1; ASH1; ASH-1; bHLHa46; class A basic helix-loop-helix protein 46; HASH1; MASH1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP50553
  • Protein Name
    Achaete-scute homolog 1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetASCL1
  • SizePrice
Western Blot Positive WB detected in: Rat brain tissue All lanes: ASCL1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 26 kDa Observed band size: 26 kDa
Product group Antibodies
Cusabio
ASCL1 AntibodyCSB-PA002199LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Rat
TargetASCL1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
TargetASCL1
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetASCL1
  • SizePrice
Product group Antibodies
Absolute Antibody
ApplicationsELISA, ImmunoHistoChemistry
TargetASCL1
  • SizePrice
Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Product group Antibodies
Atlas Antibodies
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetASCL1
  • SizePrice
Product group Antibodies
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
TargetASCL1
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetASCL1
  • SizePrice
Figure 1. Western blot analysis of ASCL1 using anti-ASCL1 antibody (A03023-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human NCL-H460 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASCL1 antigen affinity purified polyclonal antibody (Catalog # A03023-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASCL1 at approximately 30 kDa. The expected band size for ASCL1 is at 25 kDa.
Product group Antibodies
Boster Bio
Anti-ASCL1 Antibody Picoband(r)A03023-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetASCL1
  • SizePrice