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Anti-ATXN3 Antibody

144-01243
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetATXN3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-ATXN3 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4287
  • Target name
    ATXN3
  • Target description
    ataxin 3
  • Target synonyms
    AT3; ataxin-3; ATX3; JOS; josephin; Machado-Joseph disease protein 1; MJD; MJD1; olivopontocerebellar ataxia 3; SCA3; spinocerebellar ataxia type 3 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54252
  • Protein Name
    Ataxin-3
  • Scientific Description
    ATXN3 Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ataxin 3 using anti-Ataxin 3 antibody (PB9423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9423) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 3 at approximately 42KD. The expected band size for Ataxin 3 is at 42KD.
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