More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Ataxin 3 Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Ataxin 3 Polyclonal Antibody, Unconjugated (bs-17208R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Ataxin 3 Polyclonal Antibody, Unconjugated (bs-17208R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Ataxin 3 Polyclonal Antibody, Unconjugated (bs-17208R) at 1:200 overnight at 4°C, DAB staining.

Ataxin 3 Polyclonal Antibody

BS-17208R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATXN3
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    Ataxin 3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4287
  • Target name
    ATXN3
  • Target description
    ataxin 3
  • Target synonyms
    AT3, ATX3, JOS, MJD, MJD1, SCA3, ataxin-3, Machado-Joseph disease protein 1, josephin, olivopontocerebellar ataxia 3, spinocerebellar ataxia type 3 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP54252
  • Protein Name
    Ataxin-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Western blot All lanes: ATXN3 antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: Mouse brain tissue Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 42, 36, 40, 21 kDa Observed band size: 42 kDa
Product group Antibodies
Cusabio
ATXN3 AntibodyCSB-PA002443LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetATXN3
  • SizePrice
Product group Antibodies
MedChem Express
Ataxin 3 Antibody (YA1294, Rabbit)HY-P81549
TargetATXN3
  • SizePrice
Product group Antibodies
GeneTex
Ataxin 3 antibodyGTX101343
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
TargetATXN3
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ATXN3 Antibody144-01243
ApplicationsImmunoFluorescence, Western Blot
TargetATXN3
  • SizePrice
Product group Antibodies
Biosynth
Ataxin 3 antibody70R-13630
  • SizePrice
Figure 1. Western blot analysis of Ataxin 3 using anti-Ataxin 3 antibody (PB9423). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Brain Tissue Lysate Lane 2: COLO320 Whole Cell Lysate Lane 3: HELA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ataxin 3 antigen affinity purified polyclonal antibody (Catalog # PB9423) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ataxin 3 at approximately 42KD. The expected band size for Ataxin 3 is at 42KD.
Product group Antibodies
Boster Bio
Anti-Ataxin 3/ATXN3 Antibody Picoband(r)PB9423-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetATXN3
  • SizePrice
Anti-ATXN3 Antibody
Product group Antibodies
Antibodies.com
Anti-ATXN3 AntibodyA121608
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ATXN3 / JOS AntibodyLS-C763485
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetATXN3
  • SizePrice
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus & nucleoli.
Product group Antibodies
Atlas Antibodies
Anti-ATXN3 AntibodyHPA069338
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetATXN3
  • SizePrice
Product group Antibodies
Assay Genie
NOX2/gp91phox AntibodyCAB12430
TargetATXN3
  • SizePrice