
Wild-type (WT) and ATXN3 knockout (KO) 293T cell extracts (30 μg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody (GTX101343) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.<br><I>Data provided by?YCharOS?Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.</I>
Ataxin 3 antibody
GTX101343
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetATXN3
Overview
- SupplierGeneTex
- Product NameAtaxin 3 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.55 mg/ml
- ConjugateUnconjugated
- Gene ID4287
- Target nameATXN3
- Target descriptionataxin 3
- Target synonymsAT3, ATX3, JOS, MJD, MJD1, SCA3, ataxin-3, Machado-Joseph disease protein 1, josephin, olivopontocerebellar ataxia 3, spinocerebellar ataxia type 3 protein
- HostRabbit
- IsotypeIgG
- Protein IDP54252
- Protein NameAtaxin-3
- Scientific DescriptionMachado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203










![Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637658/GTX637658_T-44837_20221118_WB_M_R_22112219_124.webp)