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BAFFR Antibody (YA4437, Mouse)

HY-P84740
MedChem Express
TargetTNFRSF13C
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    BAFFR Antibody (YA4437, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID115650
  • Target name
    TNFRSF13C
  • Target description
    TNF receptor superfamily member 13C
  • Target synonyms
    BAFF-R, BAFFR, BROMIX, CD268, CVID4, prolixin, tumor necrosis factor receptor superfamily member 13C, B cell-activating factor receptor, BAFF receptor, BLyS receptor 3
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    BAFFR Antibody (YA4437) is a mouse-derived and non-conjugated IgG2b antibody, targeting to CD268. It can be applicated for WB, FC, ELISA assays, in the background of human, rat.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of BAFF Receptor using anti-BAFF Receptor antibody (A02865-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF Receptor antigen affinity purified polyclonal antibody (Catalog # A02865-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF Receptor at approximately 19KD. The expected band size for BAFF Receptor is at 19KD.
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