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Anti-BMAL1 [ARC1101] Monoclonal Antibody

CAB4714
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityRat
TargetBMAL1
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-BMAL1 [ARC1101] Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ARC1101
  • Conjugate
    Unconjugated
  • Gene ID406
  • Target name
    BMAL1
  • Target description
    basic helix-loop-helix ARNT like 1
  • Target synonyms
    ARNTL, ARNTL1, BMAL1c, JAP3, MOP3, PASD3, TIC, bHLHe5, basic helix-loop-helix ARNT-like protein 1, ARNT-like protein 1, brain and muscle, PAS domain containing 3, PAS domain-containing protein 3, aryl hydrocarbon receptor nuclear translocator like, aryl hydrocarbon receptor nuclear translocator-like protein 1, bHLH-PAS protein JAP3, basic helix-loop-helix family member e5, basic-helix-loop-helix-PAS orphan MOP3, basic-helix-loop-helix-PAS protein MOP3, brain and muscle ARNT-like 1, class E basic helix-loop-helix protein 5, member of PAS protein 3, member of PAS superfamily 3, mutant basic helix-loop-helix ARNT-like protein 1, testis tissue sperm-binding protein Li 50e
  • Host
    Rabbit
  • Protein IDO00327
  • Protein Name
    Basic helix-loop-helix ARNT-like protein 1
  • Scientific Description
    BMAL1 Monoclonal Antibody is a premium monoclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse,Rat samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Rat
  • Reactivity Supplier
    Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of BMAL1/ARNTL using anti-BMAL1/ARNTL antibody (A00260-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMAL1/ARNTL antigen affinity purified polyclonal antibody (Catalog # A00260-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMAL1/ARNTL at approximately 75 kDa. The expected band size for BMAL1/ARNTL is at 69 kDa.
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