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Anti-Caspase-3 [4F-6]

AB04219-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA, Neutralisation/Blocking
Product group Antibodies
TargetCASP3
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Caspase-3 [4F-6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis (EC50= 0.09956microg/mL). The antibody detected Caspase-3 by western blot analysis. The original format of the antibody could inhibit the activity of Caspase-3 protein in cells, inhibit cell apoptosis, and prolong the cryopreservation of the cells cycle (CN116270413A).
  • Applications
    Western Blot, ELISA, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4F-6
  • Gene ID836
  • Target name
    CASP3
  • Target description
    caspase 3
  • Target synonyms
    CPP32, CPP32B, SCA-1, caspase-3, CASP-3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1, apopain, caspase 3, apoptosis-related cysteine peptidase, caspase 3, apoptosis-related cysteine protease, cysteine protease CPP32, procaspase3, protein Yama
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP42574
  • Protein Name
    Caspase-3
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Caspase-3(P17)/CASP3 using anti-Caspase-3(P17)/CASP3 antibody (A00334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3(P17)/CASP3 antigen affinity purified polyclonal antibody (Catalog # A00334-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3(P17)/CASP3 at approximately 32 kDa. The expected band size for Caspase-3(P17)/CASP3 is at 32 kDa.
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