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Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 3 Polyclonal Antibody (bs-0081R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 3 Polyclonal Antibody (bs-0081R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
Image provided by One World Lab validation program. A431 cells probed with Rabbit Anti-Caspase 3 Polyclonal Antibody (bs-0081R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Caspase-3 Polyclonal Antibody

BS-0081R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCASP3
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Overview

  • Supplier
    Bioss
  • Product Name
    Caspase-3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID836
  • Target name
    CASP3
  • Target description
    caspase 3
  • Target synonyms
    CPP32, CPP32B, SCA-1, caspase-3, CASP-3, CPP-32, PARP cleavage protease, SREBP cleavage activity 1, apopain, caspase 3, apoptosis-related cysteine peptidase, caspase 3, apoptosis-related cysteine protease, cysteine protease CPP32, procaspase3, protein Yama
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP42574
  • Protein Name
    Caspase-3
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Tan J, Qin X, Liu B, et al. Integrative findings indicate anti-tumor biotargets and molecular mechanisms of calycosin against osteosarcoma. Biomed Pharmacother. 2020,126:110096. doi: 10.1016/j.biopha.2020.110096
    Read this paper
  • Muhammad T, Sakhawat A, Khan AA, et al. Aloperine in combination with therapeutic adenoviral vector synergistically suppressed the growth of non-small cell lung cancer. J Cancer Res Clin Oncol. 2020,146(4):861-874. doi: 10.1007/s00432-020-03157-2
    Read this paper
  • Chen S, Yu L, Zhao Q, et al. Comparative assessment of the biocompatibility and degradation behavior of Zn-3Cu and JDBM alloys used for biliary surgery. Am J Transl Res. 2020,12(1):19-31.
    Read this paper
  • Zhang M, Chen W, Zong Y, et al. Cognitive-enhancing effects of fibrauretine on Aβ(1-42)-induced Alzheimer's disease by compatibilization with ginsenosides. Neuropeptides. 2020,82:102020. doi: 10.1016/j.npep.2020.102020
    Read this paper
  • Zhang Y, Sun L, Sun Y, et al. Overexpressed CES2 has prognostic value in CRC and knockdown CES2 reverses L-OHP-resistance in CRC cells by inhibition of the PI3K signaling pathway. Exp Cell Res. 2020,389(1):111856. doi: 10.1016/j.yexcr.2020.111856
    Read this paper
  • Shen S, Lu H, Liu L, et al. Role of CD44 in tumor-initiating cells of salivary gland pleomorphic adenoma: More than a surface biomarker. Oral Dis. 2020,26(3):547-557. doi: 10.1111/odi.13279
    Read this paper
  • Li Z, Guo D, Yin X, et al. Zinc oxide nanoparticles induce human multiple myeloma cell death via reactive oxygen species and Cyt-C/Apaf-1/Caspase-9/Caspase-3 signaling pathway in vitro. Biomed Pharmacother. 2020,122:109712. doi: 10.1016/j.biopha.2019.109712
    Read this paper
  • Wan F, Zhong G, Ning Z, et al. Long-term exposure to copper induces autophagy and apoptosis through oxidative stress in rat kidneys. Ecotoxicol Environ Saf. 2020,190:110158. doi: 10.1016/j.ecoenv.2019.110158
    Read this paper
  • Yan Y, Liu X, Gao J, et al. Inhibition of TGF-β Signaling in Gliomas by the Flavonoid Diosmetin Isolated from Dracocephalum peregrinum L. Molecules. 2020,25(1). doi: 10.3390/molecules25010192
    Read this paper
  • Li YH, Jin Y, Wang XS, et al. Neuroprotective Effect of Fructus broussonetiae on APP/PS1 Mice via Upregulation of AKT/β-Catenin Signaling. Chin J Integr Med. 2021,27(2):115-124. doi: 10.1007/s11655-019-3178-4
    Read this paper

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Figure 1. Western blot analysis of Caspase-3(P17)/CASP3 using anti-Caspase-3(P17)/CASP3 antibody (A00334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3(P17)/CASP3 antigen affinity purified polyclonal antibody (Catalog # A00334-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3(P17)/CASP3 at approximately 32 kDa. The expected band size for Caspase-3(P17)/CASP3 is at 32 kDa.
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