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Goat anti-Caspase 3

EB07286
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
TargetCASP3
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Caspase 3 Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 cells. Recommended concentration: 10microg/ml. This antibody has been successfully used in ICC on Rat: Hongyi Zhao et al. (2018) PMID: 29853981.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IF/ ICC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID836
  • Target name
    CASP3
  • Target description
    caspase 3
  • Target synonyms
    apopain; CASP-3; caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; caspase-3; CPP32; CPP-32; CPP32B; cysteine protease CPP32; PARP cleavage protease; procaspase3; protein Yama; SCA-1; SREBP cleavage activity 1
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_004337.2; NP_116786.1. Purification: Antigen affinity purified. Names and symbols: CASP3; caspase 3; caspase 3, apoptosis-related cysteine protease; HGNC:1504; caspase 3, apoptosis-related cysteine peptidase; CPP32; CPP32B; SCA-1; PARP cleavage protease; SREBP cleavage activity 1; Yama; apopain; cysteine protease CPP32; procaspase3
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Troxerutin and Cerebroprotein Hydrolysate Injection Protects Neurovascular Units from Oxygen-Glucose Deprivation and Reoxygenation-Induced Injury In Vitro. Zhao H et al., 2018, Evid Based Complement Alternat Med
    Read more

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Figure 1. Western blot analysis of Caspase-3(P17)/CASP3 using anti-Caspase-3(P17)/CASP3 antibody (A00334-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Caspase-3(P17)/CASP3 antigen affinity purified polyclonal antibody (Catalog # A00334-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Caspase-3(P17)/CASP3 at approximately 32 kDa. The expected band size for Caspase-3(P17)/CASP3 is at 32 kDa.
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