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Immunohistochemical staining of human placenta shows strong nuclear positivity in decidual cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in decidual cells.
Immunohistochemical staining of human placenta shows strong nuclear positivity in decidual cells.

Anti-CCNE1 Antibody

HPA018169
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCCNE1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCNE1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID898
  • Target name
    CCNE1
  • Target description
    cyclin E1
  • Target synonyms
    CCNE; G1/S-specific cyclin-E1; pCCNE1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP24864
  • Protein Name
    G1/S-specific cyclin-E1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PANC-1 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 antigen affinity purified polyclonal antibody (Catalog # A00543-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNE1 at approximately 47KD. The expected band size for CCNE1 is at 47KD.
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