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Cyclin E1 Antibody (YA483, Rabbit)

HY-P80100
MedChem Express
TargetCCNE1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Cyclin E1 Antibody (YA483, Rabbit)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID898
  • Target name
    CCNE1
  • Target description
    cyclin E1
  • Target synonyms
    CCNE, pCCNE1, G1/S-specific cyclin-E1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Cyclin E1 Antibody (YA483) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Cyclin E1.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCNE1 using anti-CCNE1 antibody (A00543-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human U2OS whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human PANC-1 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCNE1 antigen affinity purified polyclonal antibody (Catalog # A00543-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCNE1 at approximately 47KD. The expected band size for CCNE1 is at 47KD.
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