Anti-CD123 [7G3]

Absolute Antibody

Anti-CD123 [7G3]

7

This antagonist antibody binds IL-3Ralpha and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R alpha chain expressed by transfected cells and bound to primary cells expressing IL-3Ralpha. The expression of IL-3Ralpha chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Ralpha and betac dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38- cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcgammaRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514).

Flow Cytometry, ImmunoPrecipitation, Western Blot, Neutralisation/Blocking

FC; IP; WB; FC; Block

Research Use Only

Monoclonal

7G3

IL3RA

interleukin 3 receptor subunit alpha

CD123; CD123 antigen; hIL-3Ra; IL-3 receptor subunit alpha; IL3R; IL-3R subunit alpha; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha

Human

IgG1

Interleukin-3 receptor subunit alpha

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

The original antibody was generated by intraperitoneally immunizing BALB/c mice with 5 X lo6 COS cells transfected with the IL-3R a-chain in combination with adjuvant peptides.

-20°C,2°C to 8°C

12352203