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Anti-IL3RA Antibody

144-03926
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
TargetIL3RA
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-IL3RA Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3563
  • Target name
    IL3RA
  • Target description
    interleukin 3 receptor subunit alpha
  • Target synonyms
    CD123, IL-3R-alpha, IL3R, IL3RAY, IL3RX, IL3RY, hIL-3Ra, interleukin-3 receptor subunit alpha, CD123 antigen, IL-3 receptor subunit alpha, IL-3R subunit alpha, interleukin 3 receptor, alpha (low affinity)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26951
  • Protein Name
    Interleukin-3 receptor subunit alpha
  • Scientific Description
    IL3RA Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL3RA using anti-IL3RA antibody (A04307). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Raji whole cell lysates, Lane 2: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL3RA antigen affinity purified polyclonal antibody (Catalog # A04307) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL3RA at approximately 60KD. The expected band size for IL3RA is at 60KD.
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Human U937 cells probed with IL-3R alpha Polyclonal Antibody, Unconjugated (bs-2600R) (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange).
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