Anti-CD123 [7G3]

Absolute Antibody

Anti-CD123 [7G3]

9

This antibody specifically binds to the IL-3R alpha-chain and completely abolishes its function. IL-3R is expressed on myeloid, lymphoid, and vascular endothelial cells, where it transduces IL-3-dependent signals leading to cell activation.

This antagonist antibody binds IL-3Ralpha and blocks IL-3 binding and IL-3-mediated functions. mAb 7G3 immunoprecipitated and recognized in western blots the IL-3R alpha chain expressed by transfected cells and bound to primary cells expressing IL-3Ralpha. The expression of IL-3Ralpha chain on various cells types was analysed by flow cytometry using this antibody (PMID: 8547680). It is reported that 7G3 inhibits IL-3Ralpha and betac dimerization (PMID: 8649415). This antibody was used in the study of neutrophils expressing IL-3 receptors which are activated by granulocyte-macrophage colony-stimulating factor or GM-CSF (PMID: 7579364). The original 7G3, mouse IgG2a antibody inhibited IL-3-mediated intracellular signaling of isolated acute myeloid leukemia (AML) CD34+ CD38- cells in vitro and reduced their survival (PMID: 19570512). Humanized version of this antibody CSL362 is available on request. CSL362, retains the ability to neutralise IL-3 and has enhanced affinity for the FcgammaRIIIa (CD16) on NK cells. In vitro studies have demonstrated that the increased affinity for CD16 correlates with greater antibody-dependent cell-mediated cytotoxicity (ADCC) against CD123 expressing cell lines (Busfield et al., 2012). CSL362 is being accessed for use in the treatment of myeloid leukemia (PMID: 24363400, 2524888, 26130514).

Flow Cytometry, ImmunoPrecipitation, Western Blot, Neutralisation/Blocking

Research Use Only

Monoclonal

7G3

IL3RA

interleukin 3 receptor subunit alpha

CD123; CD123 antigen; hIL-3Ra; IL-3 receptor subunit alpha; IL3R; IL-3R subunit alpha; IL-3RA; IL-3R-alpha; IL3RAY; IL3RX; IL3RY; interleukin 3 receptor, alpha (low affinity); interleukin-3 receptor subunit alpha

Human

IgG1

Interleukin-3 receptor subunit alpha

This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Human

-20°C,2°C to 8°C

12352203