Anti-CD64 [H22]

Absolute Antibody

Anti-CD64 [H22]

7

This antibody is specific for human CD64, specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by the fusion of the m22(scFv) coding regions to the truncated ETA coding sequences.

This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).

Flow Cytometry, ELISA, Other Application

CM-ELISA; FC; toxicity assays; competition studies

Research Use Only

Monoclonal

H22

FCGR1A

Fc fragment of IgG receptor Ia

CD64; CD64A; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor Ia; Fc-gamma receptor I A1; Fc-gamma RI; fc-gamma RIA; FcgammaRI; fcgammaRIa; FCRI; high affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I

Mouse

IgG1

High affinity immunoglobulin gamma Fc receptor I

This is a reformatted Fc Silent™ chimeric mouse IgG1 antibody based on the original human IgG1 antibody, created for improved compatibility with existing reagents, assays, and techniques.

Human

Human

This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA'􏳑).

-20°C,2°C to 8°C

12352203