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Anti-CD64 [H22]

Ab00731-1.4
Absolute Antibody
ApplicationsFlow Cytometry, ELISA, Other Application
Product group Antibodies
TargetFCGR1A
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD64 [H22]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds human CD64 and can be bound to a toxin so that together it displays specific cytotoxicity, and thus can be used to selectively eliminate AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur, 2003). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur, 2011).
  • Applications
    Flow Cytometry, ELISA, Other Application
  • Applications Supplier
    CM-ELISA; FC; toxicity assays; competition studies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    H22
  • Gene ID2209
  • Target name
    FCGR1A
  • Target description
    Fc fragment of IgG receptor Ia
  • Target synonyms
    CD64; CD64A; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor Ia; Fc-gamma receptor I A1; Fc-gamma RI; fc-gamma RIA; FcgammaRI; fcgammaRIa; FCRI; high affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP12314
  • Protein Name
    High affinity immunoglobulin gamma Fc receptor I
  • Scientific Description
    This is a reformatted Fc Silent™ chimeric mouse IgG1 antibody based on the original human IgG1 antibody, created for improved compatibility with existing reagents, assays, and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA'􏳑).
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of FCGR1A using anti-FCGR1A antibody (A02428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR1A at approximately 70 kDa. The expected band size for FCGR1A is at 43 kDa.
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