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ProductsBack/Cluster Of Differentiation 64 (CD64) Polyclonal Antibody

Cluster Of Differentiation 64 (CD64) Polyclonal Antibody

CAU25682
Biomatik
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetFCGR1A
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Overview

  • Supplier
    Biomatik
  • Product Name
    Cluster Of Differentiation 64 (CD64) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB; ICC; IHC-P; IHC-F; ELISA.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2209
  • Target name
    FCGR1A
  • Target description
    Fc gamma receptor Ia
  • Target synonyms
    CD64, CD64A, FCG1, FCGR1, FCRI, FcgammaRI, IGFR1, high affinity immunoglobulin gamma Fc receptor I, Fc fragment of IgG receptor Ia, Fc fragment of IgG, high affinity Ia, receptor (CD64), Fc fragment of IgG, high affinity Ia, receptor for (CD64), Fc-gamma RI, Fc-gamma receptor I A1, IgG Fc receptor I, fc-gamma RIA, fcgammaRIa
  • Host
    Rabbit
  • Protein IDP12314
  • Protein Name
    High affinity immunoglobulin gamma Fc receptor I
  • Scientific Description
    The Cluster Of Differentiation 64 (CD64) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, ICC, IHC-P, IHC-F, ELISA.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: U937 cell lysates probed with FCGR1A/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of FCGR1A using anti-FCGR1A antibody (A02428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR1A at approximately 70 kDa. The expected band size for FCGR1A is at 43 kDa.
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