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Anti-FCGR1A Antibody

144-60077
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetFCGR1A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-FCGR1A Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2209
  • Target name
    FCGR1A
  • Target description
    Fc fragment of IgG receptor Ia
  • Target synonyms
    CD64; CD64A; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc fragment of IgG, high affinity Ia, receptor for (CD64); Fc gamma receptor Ia; Fc-gamma receptor I A1; Fc-gamma RI; fc-gamma RIA; FcgammaRI; fcgammaRIa; FCRI; high affinity immunoglobulin gamma Fc receptor I; IGFR1; IgG Fc receptor I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12314
  • Protein Name
    High affinity immunoglobulin gamma Fc receptor I
  • Scientific Description
    FCGR1A Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: U937 cell lysates probed with FCGR1A/CD64 Polyclonal Antibody, Unconjugated (bs-3511R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of FCGR1A using anti-FCGR1A antibody (A02428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HCCT tissue lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FCGR1A antigen affinity purified polyclonal antibody (Catalog # A02428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FCGR1A at approximately 70 kDa. The expected band size for FCGR1A is at 43 kDa.
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