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ProductsBack/Anti-CD81 [1D6]

Anti-CD81 [1D6]

Ab00697-1.1
Absolute Antibody
ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCD81
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD81 [1D6]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is a strong inducer of homotypic adhesion in hematolymphoid cells (Levy, 1998). This antibody also has an anti-proliferative effect, and is able to induce growth inhibition of B cell lines. Triggering of CD81 by this antibody induces a significant upregulation of the constituitive release of TNF-alpha by JY cells, an Eptsein-Barr virus-positive B lymphoblastoid cell line (Altomonte, 1995).
  • Applications
    Functional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Functional; ELISA; FC; ICC/IF; IHC-P; IP; WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D6
  • Gene ID975
  • Target name
    CD81
  • Target description
    CD81 molecule
  • Target synonyms
    26 kDa cell surface protein TAPA-1; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); CVID6; S5.7; TAPA1; tetraspanin-28; TSPAN28; tspan-28
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP60033
  • Protein Name
    CD81 antigen
  • Reactivity Supplier
    Human, Chimpanzee, Goat, Sheep
  • Reactivity Supplier Note
    This antibody was produced by immunizing mice with 5A6-aggregated OCI-LY8 B cells.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse raw264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.
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