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CD81 antibody

10R-CD81DMS
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    CD81 antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    41116161

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IHC image of CSB-PA004960NA01HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse raw264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.
Product group Antibodies
Boster Bio
Anti-TAPA1/CD81 Antibody Picoband(r)A01281-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityHuman, Mouse, Rat
TargetCD81
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Product group Antibodies
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ApplicationsFunctional Assay, Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityGoat, Human, Primate, Sheep
TargetCD81
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Anti-CD81 Antibody
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ReactivityHuman, Mouse, Rat
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Product group Antibodies
MedChem Express
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TargetCD81
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Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA007234 antibody. Corresponding CD81 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
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ReactivityHuman
TargetCD81
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Product group Antibodies
LifeSpan BioSciences
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Mouse spleen lysates probed with CD81 Polyclonal Antibody, Unconjugated (bs-6934R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Product group Antibodies
Bioss
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ReactivityHuman, Mouse, Rat
TargetCD81
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Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CD81 antibody (GTX101766) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
CD81 antibodyGTX101766
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
ReactivityHuman, Mouse, Rat
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Product group Antibodies
RayBiotech
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TargetCD81
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