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CD81 antibody

GTX101766
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
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Product group Antibodies
TargetCD81
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD81 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID975
  • Target name
    CD81
  • Target description
    CD81 molecule
  • Target synonyms
    26 kDa cell surface protein TAPA-1; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); CVID6; S5.7; TAPA1; tetraspanin-28; TSPAN28; tspan-28
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60033
  • Protein Name
    CD81 antigen
  • Scientific Description
    The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Adipose mesenchymal stem cell-derived extracellular vesicles reduce glutamate-induced excitotoxicity in the retina.
    Read more
  • Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axis.
    Read more
  • Oral Administration of Bovine Milk-Derived Extracellular Vesicles Attenuates Cartilage Degeneration via Modulating Gut Microbiota in DMM-Induced Mice.
    Read more
  • Machine learning-assisted elucidation of CD81-CD44 interactions in promoting cancer stemness and extracellular vesicle integrity.
    Read more
  • miR-29a-3p/THBS2 Axis Regulates PAH-Induced Cardiac Fibrosis. Hsu CH et al., 2021 Sep 30, Int J Mol Sci
    Read more
  • Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota. Tong L et al., 2021, Theranostics
    Read more
  • Involvement of Circulating Exosomal MicroRNAs in Jian-Pi-Yi-Shen Formula Protection Against Adenine-Induced Chronic Kidney Disease. Liu X et al., 2020, Front Pharmacol
    Read more
  • Comprehensive analysis and comparison of proteins in salivary exosomes of climacteric and adolescent females. Imai A et al., 2021 Jan, Odontology
    Read more
  • Phosphorylated Ezrin (Thr567) Regulates Hippo Pathway and Yes-Associated Protein (Yap) in Liver. Xue Y et al., 2020 Jul, Am J Pathol
    Read more
  • Oral Administration of Bovine Milk-Derived Extracellular Vesicles Alters the Gut Microbiota and Enhances Intestinal Immunity in Mice. Tong L et al., 2020 Apr, Mol Nutr Food Res
    Read more

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Figure 1. Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse raw264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.
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