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ProductsBack/CD81 Antibody (PE)

CD81 Antibody (PE)

LS-C485188
LifeSpan BioSciences
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD81
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    CD81 Antibody (PE)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry
  • Applications Supplier
    Flo
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    RPE
  • Estimated Purity
    ...
  • Gene ID975
  • Target name
    CD81
  • Target description
    CD81 molecule
  • Target synonyms
    26 kDa cell surface protein TAPA-1; CD81 antigen; CD81 antigen (target of antiproliferative antibody 1); CVID6; S5.7; TAPA1; tetraspanin-28; TSPAN28; tspan-28
  • Host
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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IHC image of CSB-PA004960NA01HU diluted at 1:100 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of TAPA1 using anti-TAPA1 antibody (A01281-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse raw264.7 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAPA1 antigen affinity purified polyclonal antibody (Catalog # A01281-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAPA1 at approximately 22KD. The expected band size for TAPA1 is at 22KD.
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